Description

R-loops are unusual nucleic acid structures consisting of an RNA/DNA hybrid and a single stranded DNA (ssDNA). They form during transcription and regulate key physiological processes, including gene expression. However, R-loop imbalance, caused by their unscheduled formation or failure to be timely resolved, can lead to genome instability and deregulated gene expression and has been involved in oncogenesis and cancer progression. To assess R-loop contribution to these processes, accurate R-loop detection and quantification in cancer contexts is necessary.

Our project aims to develop and apply structure-specific antibodies to quantitatively detect R-loops and use them to monitor R-loop dynamics in cancer cell lines. To achieve this, we will characterize specific candidate antibodies that we have generated. Selected candidates will be validated in vitro and in cells, in comparison with reagents currently used in the field. They will then ...